Coagulase testing compared with commercial kits for routinely identifying Staphylococcus aureus.

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Coagulase testing compared with commercial kits for routinely identifying Staphylococcus aureus.

Five commercial Staphylococcus aureus identification kits--Staphaurex (Wellcome), Staphylase (Oxoid), Staphyslide (bioMèrieux), Biostaph (Medlabs) and Bacto Latex (Difco)--were evaluated for the routine identification of S aureus from primary plates in the routine microbiology laboratory. Comparison was made with two methods of tube coagulase testing and five slide methods for detecting clumpin...

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Comparison of commercial slide agglutination kits with a tube coagulase test for the rapid identification of Staphylococcus aureus from blood culture.

Eighty clinical specimens of BACTEC 9240 blood culture vials, culture positive for staphylococci (38 Staphylococcus aureus and 42 coagulase negative staphylococci), were tested directly for the presence of clumping factor/protein A and free coagulase. Seven commercial slide agglutination kits were compared with a direct-tube coagulase (DTC) method. All tests were performed on blood culture pell...

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Homology Modeling of Coagulase in Staphylococcus aureus

The close correlation between the ability of coagulase to clot blood plasma and their capacity to produce disease, and the corresponding absence of this property in nonpathogenic strains, have led to the assumption that the coagulase, plays important role in the pathogenesis of disease. Currently, crystal structure of coagulase in Staphylococcus aureus remains indefinable. Thus, the objectives ...

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Simultaneous Identification of Staphylococcus aureus and Coagulase

22 A novel rapid PNA FISH method, Staphylococcus QuickFISH, for the direct 23 detection of Staphylococcus species from positive blood culture bottles was 24 evaluated in a multicenter clinical study. The method utilizes a microscope slide 25 with pre-deposited positive and negative control organisms and a self-reporting 26 15 minute hybridization step which eliminates the need for a wash step. ...

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Variation of bound coagulase of Staphylococcus aureus.

Duthie (1954) reported that bound coagulase (clumping factor) of staphylococei is released from the surface of autolysing cells into the medium of old broth cultures. Rosenblum and Jackson (1957) noticed occasional alteration of bound coagulase and pigment production after storage of staphylococci for months to years. Decrease in bound coagulase activity of freshly isolated hospital strains of ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1990

ISSN: 0021-9746

DOI: 10.1136/jcp.43.3.246